2YX9
Crystal structure of D298K copper amine oxidase from Arthrobacter globiformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-02 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 158.012, 63.027, 184.119 |
| Unit cell angles | 90.00, 111.96, 90.00 |
Refinement procedure
| Resolution | 39.880 - 1.680 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.042 | 0.338 |
| Number of reflections | 190511 | |
| Completeness [%] | 99.7 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | 1.05M potassium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |
