2YX8

Crystal structure of the extracellular domain of human RAMP1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-26
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9789, 0.9793, 0.9640
Spacegroup name	P 42 2 2
Unit cell lengths	43.097, 43.097, 82.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.560 - 2.400
Rwork	0.221
R-free	0.28100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	3371
<I/σ(I)>	21.26	2.59
Completeness [%]	99.3	93.2
Redundancy	15.15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	0.1M tri-Sodium Citrate Dihydrate, 0.15M di-Ammonium Citrate, 25% PEG3350, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K