2YX2

Crystal structure of cloned trimeric hyluranidase from streptococcus pyogenes at 2.8 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	298
Detector technology	CCD
Collection date	2006-11-20
Detector	MARRESEARCH
Wavelength(s)	0.803
Spacegroup name	H 3 2
Unit cell lengths	58.770, 58.770, 586.241
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.19471
Rwork	0.192
R-free	0.22397
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c3f
RMSD bond length	0.016
RMSD bond angle	1.645
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.840
High resolution limit [Å]	2.800	2.800
Number of reflections	10238
<I/σ(I)>	6.7	2.1
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	TRIS HCL, SODIUM FORMATE, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K