2YWZ
Structure of new antigen receptor variable domain from sharks
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-20 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.252, 50.442, 46.731 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 18.110 - 2.210 |
| R-factor | 0.18853 |
| Rwork | 0.185 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ves |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.593 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.110 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.008 | 0.550 |
| Number of reflections | 6619 | |
| <I/σ(I)> | 21 | 2.8 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M CACODYLATE, 36% MPEG 2000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
