2YWY
Structure of new antigen receptor variable domain from sharks
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-05 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.498, 88.661, 101.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.570 - 2.710 |
| R-factor | 0.189 |
| Rwork | 0.180 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ves |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.547 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.570 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.012 | 0.600 |
| Number of reflections | 19373 | |
| <I/σ(I)> | 7.6 | 1.2 |
| Completeness [%] | 95.3 | 87.6 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M ACETATE, 20% PEP (17/8 PO/OH), pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
