2YWQ
Crystal structure of Thermus thermophilus Protein Y N-terminal domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97909, 0.97928, 0.96405 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 81.774, 81.774, 136.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.130 - 2.640 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.29400 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.280 | 2.730 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Number of reflections | 14256 | |
| <I/σ(I)> | 28.52 | 7.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 27.84 | 28.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 4M Sodium formate, pH 8.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
