2YWM
Crystal structure of glutaredoxin-like protein from Aquifex aeolicus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-16 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9789, 0.9000, 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.490, 99.600, 105.830 |
| Unit cell angles | 90.00, 116.12, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.051 | 0.314 |
| Number of reflections | 83966 | |
| <I/σ(I)> | 26.7 | 5.11 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.9M Ammonium phosphate, 90MM Sodium acetate, 90MM Lithium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
