2YWK
Crystal structure of RRM-domain derived from human putative RNA-binding protein 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.366, 46.366, 59.596 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.300 - 1.540 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p27 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.330 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 11182 | |
| <I/σ(I)> | 42.06 | 8.98 |
| Completeness [%] | 98.1 | 96.9 |
| Redundancy | 21.61 | 22.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 1.26M Sodium dihydrogen phosphate, 0.14M di-Potassium hydrogen phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
