2YWI
Crystal structure of uncharacterized conserved protein from Geobacillus kaustophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-21 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97886, 0.9000, 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.382, 57.223, 79.526 |
| Unit cell angles | 90.00, 107.15, 90.00 |
Refinement procedure
| Resolution | 38.000 - 1.600 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 | 0.205 |
| Number of reflections | 53368 | |
| <I/σ(I)> | 13.3 | 6.19 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.4M Ammonium phosphate dibasic, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
