2YWE
Crystal structure of LepA from Aquifex aeolicus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-06-14 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.000, 0.9642, 0.9793, 0.9794 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.570, 115.836, 123.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.180 - 2.050 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.180 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 36651 | |
| <I/σ(I)> | 26.95 | 2.27 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.13 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M Bis-Tris, 0.1M MgCl2, 20% w/v PEG 3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
