2YW9
Crystal structure of TT0143 from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-11-29 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.087, 107.131, 184.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.22644 |
| Rwork | 0.222 |
| R-free | 0.26273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ulu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.3.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.876 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.131 | 0.485 |
| Number of reflections | 66626 | |
| <I/σ(I)> | 4.5 | 1.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG4000, LiCl, Sodium Citerate, NaCl, NADP(+), pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
