2YW7
Crystal structure of C-terminal deletion mutant of Mycobacterium smegmatis Dps
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-20 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.516, 83.940, 111.911 |
| Unit cell angles | 90.00, 105.89, 90.00 |
Refinement procedure
| Resolution | 28.540 - 3.300 |
| R-factor | 0.22367 |
| Rwork | 0.222 |
| R-free | 0.25878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vei |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.068 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.382 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.011 | 0.033 |
| Number of reflections | 24653 | |
| <I/σ(I)> | 11.7 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG1140, 100mM CaCl2, 0.1M Tris-HCl pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
