2YW6
Structural studies of N terminal deletion mutant of Dps from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-22 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 87.443, 87.443, 212.537 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.620 - 2.530 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vei |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.098 | 0.497 |
| Number of reflections | 15936 | |
| <I/σ(I)> | 28 | 3.83 |
| Completeness [%] | 94.4 | 88.3 |
| Redundancy | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbath under oil | 8 | 293 | protein buffer in 50mM Tris-HCl (pH8.0), 0.2M Calcium chloride, 0.1M HEPES-Na pH7.5, 28% polyethelene glycol 400, Microbath under oil, temperature 293K |
