2YVY

Crystal structure of magnesium transporter MgtE cytosolic domain, Mg2+ bound form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97911, 0.97922, 0.97700
Spacegroup name	P 65 2 2
Unit cell lengths	57.727, 57.727, 317.561
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.310 - 2.300
R-factor	0.256
Rwork	0.256
R-free	0.28600
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	14931
<I/σ(I)>	13.7	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	33% PEG 400, 0.2M MgCl2, 0.1M HEPES, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K