2YVU
Crystal structure of APE1195
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-28 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.010, 102.820, 45.580 |
| Unit cell angles | 90.00, 96.67, 90.00 |
Refinement procedure
| Resolution | 45.270 - 2.100 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gks |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.045 | 0.159 |
| Number of reflections | 23213 | |
| <I/σ(I)> | 31.2 | 5.4 |
| Completeness [%] | 98.8 | 91.3 |
| Redundancy | 5.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil batch | 7.6 | 293 | 15% (v/v) MPD, 0.1M HEPES-NaOH, 0.2M tri-Na Citr, pH 7.6, oil batch, temperature 293K |
