2YVQ
Crystal structure of MGS domain of carbamoyl-phosphate synthetase from homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.505, 50.505, 97.634 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.110 - 1.980 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 10442 | |
| <I/σ(I)> | 37.2 | 8.25 |
| Completeness [%] | 94.5 | 94.5 |
| Redundancy | 10.7466 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M (NH4)2SO4, 0.1M MES, 10%(V/V) dioxane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
