2YVI

Crystal structure of a death domain of human ankryn protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9782
Spacegroup name	P 61 2 2
Unit cell lengths	46.630, 46.630, 145.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.990 - 1.920
R-factor	0.198
Rwork	0.198
R-free	0.21700
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.990
High resolution limit [Å]	1.920	1.920
Rmerge		0.187
Number of reflections	7800
Completeness [%]	99.8	99.9
Redundancy	30.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2M NaCl, 0.1M TrisCl pH8.5, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K