2YVF

Crystal structure of ferredoxin reductase BPHA4 (hydroquinone)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-17
Detector	ADSC QUANTUM 210
Spacegroup name	P 61 2 2
Unit cell lengths	97.597, 97.597, 169.463
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.600
R-factor	0.189
Rwork	0.188
R-free	0.21400
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.011
RMSD bond angle	1.420
Data scaling software	XDS
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.680
High resolution limit [Å]	1.600	1.600
Rmerge	0.067	0.685
Number of reflections	60816
<I/σ(I)>	21.54	3.1
Completeness [%]	95.9	94.4
Redundancy	11.6	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	2M SODIUM FORMATE, 0.1M SODIUM ACETATE, pH 5.40, VAPOR DIFFUSION, SITTING DROP, temperature 293K