2YV7
Crystal structure of the CLIC homolog from drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.393, 63.451, 114.122 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.700 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k0m |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.765 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.070 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.059 | 0.233 |
| Number of reflections | 29963 | |
| <I/σ(I)> | 18.3 | 3.3 |
| Completeness [%] | 97.9 | 91.3 |
| Redundancy | 5.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 20% PEG 3350, pH 7.40, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
