2YV2
Crystal Structure of Succinyl-CoA Synthetase Alpha Chain from Aeropyrum pernix K1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-11 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.542, 137.183, 74.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.130 - 2.200 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oi7 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.093 | 0.343 |
Number of reflections | 15880 | |
<I/σ(I)> | 23.5 | 7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.9 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8 | 291 | 50% Ethylene glycol, 0.1M imidazole, pH8.0, Microbatch, temperature 291K |