2YV1

Crystal Structure of Succinyl-CoA Synthetase Alpha Chain from Methanocaldococcus jannaschii DSM 2661

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-19
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	65.882, 65.044, 65.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.490 - 1.700
R-factor	0.205
Rwork	0.205
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oi7
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.046	0.366
Number of reflections	31324
<I/σ(I)>	44.1	5.6
Completeness [%]	98.8	100
Redundancy	7.1	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1M Sodium Acetate, 20% PEG 3350, 0.2M Lithium Sulfate, pH5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K