2YRS
Human hemoglobin D Los Angeles: crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2003-06-26 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.4310 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.702, 62.759, 110.216 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 41.420 - 2.300 |
| R-factor | 0.16493 |
| Rwork | 0.160 |
| R-free | 0.25248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dxt |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.152 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.420 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.094 | 0.256 |
| Number of reflections | 45030 | |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
