2YR5
Crystal structure of L-phenylalanine oxidase from Psuedomonas sp.P501
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-13 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.97200 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.648, 112.624, 136.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.620 - 1.250 |
| R-factor | 0.10272 |
| Rwork | 0.101 |
| R-free | 0.12851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yr4 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.085 | 0.381 |
| Number of reflections | 429065 | |
| Completeness [%] | 97.8 | 93.4 |
| Redundancy | 5.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1M HEPES pH7.5, 1.0M ammonium sulfate, VAPOR DIFFUSION, temperature 293K |
