2YQ4

Crystal Structure of D-isomer specific 2-hydroxyacid dehydrogenase from Lactobacillus delbrueckii ssp. bulgaricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	92.211, 108.367, 147.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.997 - 3.450
R-factor	0.1828
Rwork	0.179
R-free	0.24960
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.020
RMSD bond angle	1.836
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.720
High resolution limit [Å]	3.450
Rmerge	0.170
Number of reflections	19722
<I/σ(I)>	8
Completeness [%]	98.9
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				25% PEG 3350, 200MM MGCL2, 100MM HEPES PH7.5