2YQ2
Structure of BVDV1 envelope glycoprotein E2, pH8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.180, 47.593, 159.290 |
| Unit cell angles | 90.00, 108.15, 90.00 |
Refinement procedure
| Resolution | 38.080 - 2.580 |
| R-factor | 0.2406 |
| Rwork | 0.240 |
| R-free | 0.25270 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.110 | 1.000 |
| Number of reflections | 29885 | |
| <I/σ(I)> | 27.6 | 1.7 |
| Completeness [%] | 97.0 | 76.1 |
| Redundancy | 5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 52.5% METHYL-2,4-PENTANEDIOL (MPD) AND 0.1 M TRIS PH 8.0 |
