2YPY

KSHV LANA (ORF73) C-terminal domain, decameric ring: monoclinic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-06
Detector	MARRESEARCH
Wavelength(s)	1.07208, 0.97974
Spacegroup name	P 1 21 1
Unit cell lengths	51.740, 175.920, 97.580
Unit cell angles	90.00, 95.50, 90.00

Refinement procedure

Resolution	19.652 - 2.453
R-factor	0.2224
Rwork	0.221
R-free	0.25220
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.003
RMSD bond angle	0.645
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.530
High resolution limit [Å]	2.450	2.450
Rmerge	0.050	0.480
Number of reflections	54311
<I/σ(I)>	16.24	2.6
Completeness [%]	85.7	21.7
Redundancy	3.8	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	285	1.5 UL OF 2.0 MM PROTEIN IN 5 MM BISTRIS, PH 6.5, 10 MM DTT WERE ADDED TO 1.5 UL OF 0.1 M SODIUM BICINE, PH 9.0, 1.0 M LITHIUM CHLORIDE, 7 % (W/V) PEG 6000. THE MIXTURE WAS INCUBATED AT 12 DEGREES CENTIGRADE IN A HANGING DROP SETUP. CRYSTALS GREW IN A FEW DAYS AND WERE CRYO-PROTECTED BY SHORT SOAKING IN 0.1 M SODIUM BICINE, PH 9.0, 1.0 M LITHIUM CHLORIDE, 7 % (W/V) PEG 6000, 25 % (V/V) GLYCEROL.