2YPS
Crystal structure of the PX domain of human sorting nexin 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-29 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.840, 81.700, 184.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.020 - 2.600 |
| R-factor | 0.21308 |
| Rwork | 0.211 |
| R-free | 0.25511 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2csk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.020 | 2.730 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 16601 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5M FORMATE |
