2YPS
Crystal structure of the PX domain of human sorting nexin 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-29 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.840, 81.700, 184.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.020 - 2.600 |
R-factor | 0.21308 |
Rwork | 0.211 |
R-free | 0.25511 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2csk |
RMSD bond length | 0.014 |
RMSD bond angle | 1.505 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.020 | 2.730 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.080 | 0.500 |
Number of reflections | 16601 | |
<I/σ(I)> | 8.2 | 2.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.5M FORMATE |