2YOC
Crystal structure of PulA from Klebsiella oxytoca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 179.150, 179.150, 334.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.170 - 2.880 |
| R-factor | 0.1733 |
| Rwork | 0.171 |
| R-free | 0.21310 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.640 | 3.040 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.110 | 0.670 |
| Number of reflections | 71937 | |
| <I/σ(I)> | 20.4 | 4 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 14.5 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8 M AMMONIUM SULPHATE, 2 % MPD AND 100 MM SODIUM CITRATE (PH 7.0) |
