2YO1
Salmonella enterica SadA 1049-1304 fused to GCN4 adaptors (SadAK9- cfII)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.030, 48.780, 135.830 |
| Unit cell angles | 90.00, 105.10, 90.00 |
Refinement procedure
| Resolution | 38.640 - 3.100 |
| R-factor | 0.26752 |
| Rwork | 0.265 |
| R-free | 0.32049 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 3.280 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.420 |
| Number of reflections | 18792 | |
| <I/σ(I)> | 8.93 | 2.35 |
| Completeness [%] | 96.9 | 94.6 |
| Redundancy | 2.31 | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15 % (V/V) BUTANEDIOL, 100 MM SODIUM ACETATE PH 4.2 |
