2YNZ
Salmonella enterica SadA 823-947 fused to a GCN4 adaptor (SadAK5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.690, 60.300, 135.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.270 - 1.400 |
| R-factor | 0.17925 |
| Rwork | 0.176 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.099 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.070 | 0.680 |
| Number of reflections | 71862 | |
| <I/σ(I)> | 12.3 | 1.97 |
| Completeness [%] | 98.8 | 97.9 |
| Redundancy | 4.38 | 4.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% (W/V) PEG 10000, 200 MM MAGNESIUM NITRATE |
