2YNY
Salmonella enterica SadA 255-302 fused to GCN4 adaptors (SadAK1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.810, 40.360, 98.620 |
| Unit cell angles | 90.00, 93.51, 90.00 |
Refinement procedure
| Resolution | 37.950 - 1.350 |
| R-factor | 0.13609 |
| Rwork | 0.133 |
| R-free | 0.19844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.060 | 0.630 |
| Number of reflections | 57902 | |
| <I/σ(I)> | 13.9 | 1.98 |
| Completeness [%] | 96.0 | 89.6 |
| Redundancy | 4.3 | 3.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16% (W/V) PEG 4000, 80 MM SODIUM ACETATE, 100 MM HEPES PH 7.5 |
