2YN3
Structural insight into the giant calcium-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-08 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.480, 153.309, 96.300 |
| Unit cell angles | 90.00, 103.88, 90.00 |
Refinement procedure
| Resolution | 34.320 - 2.120 |
| R-factor | 0.21271 |
| Rwork | 0.210 |
| R-free | 0.25971 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.553 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.076 | 0.396 |
| Number of reflections | 72931 | |
| <I/σ(I)> | 10.7 | 2.9 |
| Completeness [%] | 98.0 | 85.6 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM IODIDE, 20 % PEG 3350 |
