2YN2
Huf protein - paralogue of the tau55 histidine phosphatase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.720, 86.720, 98.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.360 - 2.050 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2YN0 (EDITED WITH SCULPTOR) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.913 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.070 | 0.990 |
| Number of reflections | 27487 | |
| <I/σ(I)> | 18.19 | 2.54 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.17 | 11.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 0.2 M MAGNESIUM FORMATE, 26% PEG 3,350, pH 5.9 |
