2YN1
Crystal Structure of Ancestral Thioredoxin Relative to Last Gamma- Proteobacteria Common Ancestor (LGPCA) from the Precambrian Period
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.090, 62.910, 42.910 |
| Unit cell angles | 90.00, 109.17, 90.00 |
Refinement procedure
| Resolution | 34.089 - 1.300 |
| R-factor | 0.1694 |
| Rwork | 0.168 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2trx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.090 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 43308 | |
| <I/σ(I)> | 12.47 | 2.68 |
| Completeness [%] | 97.3 | 97.8 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 3 | 277 | COUNTER-DIFFUSION TECHNIQUE. 30% PEG 400, 0.1 M TRIS-HCL, 0.2 M NA CITRATE, PH 8.50, 277K. |
