2YN0
tau55 histidine phosphatase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.580, 86.730, 44.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.365 - 1.500 |
| R-factor | 0.1573 |
| Rwork | 0.156 |
| R-free | 0.18260 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.248 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.160 | 0.990 |
| Number of reflections | 50268 | |
| <I/σ(I)> | 15.5 | 2.19 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.05 | 4.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.77 M NAH2PO4, 1.23 M K2HPO4 |
