2YMY
Structure of the murine Nore1-Sarah domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-01 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.820, 84.410, 39.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.205 - 1.690 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.23530 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.406 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.210 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.060 | 0.760 |
| Number of reflections | 13449 | |
| <I/σ(I)> | 16.3 | 2.4 |
| Completeness [%] | 99.3 | 95.4 |
| Redundancy | 6.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 25-26 % (V/V) PEG 400, 100 MM SODIUM ACETATE (PH 4.6), 45-50 MM CADMIUM CHLORIDE. |
