2YMA
X-ray structure of the Yos9 dimerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-19 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 160.800, 160.800, 97.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.793 - 2.545 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22040 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | SEMET DERIVATIVE REFINED MODEL SOLVED BY MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.800 | 2.610 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.060 | 0.600 |
| Number of reflections | 15832 | |
| <I/σ(I)> | 22.2 | 2.9 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.55 M NAH2PO4, 0.55 M KH2PO4, 0.1 M TRIS, PH 5.05. |
