2YM9
SipD from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-14 |
| Detector | MARRESEARCH SX-125 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 128.370, 128.370, 350.082 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.940 - 3.000 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j0n |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.170 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.510 |
| Number of reflections | 34082 | |
| <I/σ(I)> | 13.86 | 3.58 |
| Completeness [%] | 96.7 | 92.9 |
| Redundancy | 7.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN WAS MIXED WITH SOLUTION CONTAINING 100 MM HEPES PH 7.5 AND 1.5 M LI2SO4. |
