2YM9
SipD from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-14 |
Detector | MARRESEARCH SX-125 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 128.370, 128.370, 350.082 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.940 - 3.000 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j0n |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | CNS (1.21) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.170 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.100 | 0.510 |
Number of reflections | 34082 | |
<I/σ(I)> | 13.86 | 3.58 |
Completeness [%] | 96.7 | 92.9 |
Redundancy | 7.6 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | PROTEIN WAS MIXED WITH SOLUTION CONTAINING 100 MM HEPES PH 7.5 AND 1.5 M LI2SO4. |