2YLW
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: Arg337Lys MUTANT IN COMPLEX WITH MES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.730, 107.730, 106.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.700 - 2.900 |
| R-factor | 0.18437 |
| Rwork | 0.180 |
| R-free | 0.26552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ylr |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.023 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.050 | 0.180 |
| Number of reflections | 15840 | |
| <I/σ(I)> | 20.8 | 12.9 |
| Completeness [%] | 98.0 | 97.6 |
| Redundancy | 4.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SEE PDB ENTRY 2YLR, pH 6.5 |
