2YLI
RECOMBINANT NATIVE CYTOCHROME C PRIME FROM ALCALIGENES XYLOSOXIDANS IN ITS FERROUS FORM AT 1.45 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-16 |
| Detector | MAR |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.266, 53.266, 180.731 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.130 - 1.450 |
| R-factor | 0.15133 |
| Rwork | 0.150 |
| R-free | 0.18354 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ykz |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.772 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 27081 | |
| <I/σ(I)> | 14 | 7 |
| Completeness [%] | 94.6 | 94 |
| Redundancy | 9.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, TRIS PH 7.5 |
