2YLG

CYTOCHROME C PRIME FROM ALCALIGENES XYLOSOXIDANS: ASCORBATE AND CARBON MONOOXIDE BOUND L16A VARIANT AT 1.05 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-06-04
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 65 2 2
Unit cell lengths	53.640, 53.640, 181.443
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.450 - 1.050
R-factor	0.13846
Rwork	0.138
R-free	0.15076
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yl0
RMSD bond length	0.020
RMSD bond angle	2.042
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.100
High resolution limit [Å]	1.050	1.050
Rmerge	0.050	0.800
Number of reflections	73117
<I/σ(I)>	21.5	1.8
Completeness [%]	100.0	100
Redundancy	11	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		AMMONIUM SULPHATE, TRIS PH 7.5.