2YL6

Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Temperature [K]	77
Spacegroup name	I 2 2 2
Unit cell lengths	78.286, 108.999, 112.433
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 1.600
R-factor	0.18784
Rwork	0.186
R-free	0.21942
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.073
Data scaling software	SCALEIT
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.930	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.060	0.390
Number of reflections	59835
<I/σ(I)>	11.7	3.1
Completeness [%]	94.0	85.5
Redundancy	3.9	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1