2YL2
Crystal structure of human acetyl-CoA carboxylase 1, biotin carboxylase (BC) domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.670, 60.870, 70.370 |
| Unit cell angles | 92.04, 98.00, 92.20 |
Refinement procedure
| Resolution | 33.670 - 2.300 |
| R-factor | 0.2076 |
| Rwork | 0.206 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.770 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 43240 | |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 97.7 | 96.9 |
| Redundancy | 2.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 200 MM NA(MALONATE) PH 7.0 |
