2YKY
Structural Determinants of the Beta-Selectivity of a Bacterial Aminotransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-25 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 183.583, 94.461, 103.671 |
Unit cell angles | 90.00, 113.83, 90.00 |
Refinement procedure
Resolution | 47.420 - 1.690 |
R-factor | 0.15793 |
Rwork | 0.157 |
R-free | 0.17653 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MES_BFAT_APO |
RMSD bond length | 0.009 |
RMSD bond angle | 1.123 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.160 | 1.780 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.040 | 0.240 |
Number of reflections | 180581 | |
<I/σ(I)> | 19.1 | 4.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |