2YKX
Structural Determinants of the Beta-Selectivity of a Bacterial Aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2010-04-10 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.761, 94.422, 103.729 |
| Unit cell angles | 90.00, 113.65, 90.00 |
Refinement procedure
| Resolution | 48.230 - 1.850 |
| R-factor | 0.17715 |
| Rwork | 0.176 |
| R-free | 0.19650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MES_BFAT_HOLO |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.230 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 138206 | |
| <I/σ(I)> | 8.1 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
