2YKU
Structural Determinants of the Beta-Selectivity of a Bacterial Aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-27 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.195, 94.833, 104.892 |
| Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
| Resolution | 96.030 - 1.900 |
| R-factor | 0.16364 |
| Rwork | 0.162 |
| R-free | 0.19099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2E7U 2cy8 1ohv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER (FOR MR) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 129742 | |
| <I/σ(I)> | 12.6 | 3.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.5, 8% V/V ETHYLENE GLYCOL, 10 % W/V PEG 8K |
