2YKS
PENTAMERIC LIGAND GATED ION CHANNEL ELIC MUTANT F246A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2010-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.502, 266.147, 110.851 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 19.970 - 3.300 |
| R-factor | 0.243 |
| Rwork | 0.242 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vl0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.516 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 133.070 | 10.090 |
| High resolution limit [Å] | 3.300 | 3.380 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 140127 | |
| <I/σ(I)> | 11.08 | 1.9 |
| Completeness [%] | 88.3 | 78.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50MM ADA PH6.5, 200MM NALISO4, 10% (W/V) PEG4000 |
