2YKQ

Structure of the human LINE-1 ORF1p trimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	90
Detector technology	CCD
Collection date	2009-06-21
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	71.440, 91.640, 107.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.805 - 3.100
R-factor	0.2418
Rwork	0.239
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2YKO AND 2W7A
RMSD bond length	0.002
RMSD bond angle	0.472
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.800	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	13318
<I/σ(I)>	11.3	2.2
Completeness [%]	99.6	100
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		100 MM TRIS-CL PH 8.5, 100 MM MG-ACETATE, 12 % PEG 8000