2YKK
Structure of a Paenibacillus Polymyxa Xyloglucanase from Glycoside Hydrolase Family 44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.170, 84.170, 157.571 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 158.110 - 1.790 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e0p |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.641 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.060 | 0.160 |
| Number of reflections | 60915 | |
| <I/σ(I)> | 23.9 | 9.6 |
| Completeness [%] | 98.8 | 91.6 |
| Redundancy | 6.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% (W/V) PEG 3350, 0.2M LITHIUM SULPHATE, 0.1M BIS TRIS PH 6.5 |
