2YKH
Sensor region of a sensor histidine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.920, 79.535, 102.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.780 |
| R-factor | 0.25148 |
| Rwork | 0.248 |
| R-free | 0.29920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ykf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.412 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.180 |
| Number of reflections | 15488 | |
| <I/σ(I)> | 20 | 6.2 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
